I took these pictures last semester.
We were being taught how to do immunocytochemistry, a laboratory technique in which a specific antibody is used to detect the presence of a particular antigen or protein in a cell. Because the antibody is specific for the protein of interest, it binds the protein and allows visualization by staining and examination under a microscope. Naturally, darker staining relates to protein concentration in those particular areas.
We were given an antibody and were told to determine what protein it was specific for by staining and visualizing our samples. After a long search, I came up with Calbindin D, a calcium-binding vitamin D dependent protein which helps transport calcium. It’s localization includes the cerebellum & cerebellar cortex, the hippocampus and the dentate gyrus, as well as the striatum and other components of the basal ganglia.
